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AFLP marker analysis revealing genetic structure of the tree Parapiptadenia rigida (Benth.) Brenan (Leguminosae-Mimosoideae) in the southern Brazilian Tropical Rainforest Genet. Mol. Biol.
Souza,Laís Bérgamo de; Ruas,Eduardo A.; Rodrigues,Luana A.; Ruas,Claudete F.; Ruas,Paulo M..
Parapiptadenia rigida is a tropical early secondary succession tree characteristic of the Tropical Atlantic Rainforest. This species is of great ecological importance in the recovery of degraded areas. In this study we investigated the variability and population genetic structure of eight populations of P. rigida. Five AFLP primer combinations were used in a sample of 159 individuals representing these eight populations, rendering a total of 126 polymorphic fragments. The averages of percentage of polymorphic loci, gene diversity, and Shannon index were 60.45%, 0.217, and 0.322, respectively. A significant correlation between the population genetic variability and the population sizes was observed. The genetic variability within populations (72.20%) was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tropical tree; Genetic diversity; Population genetics; Conservation; AFLP.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400012
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Chromosome studies in some Stevia. Cav. (Compositae) species from Southern Brazil Braz. J. Genet.
Frederico,Adriana P.; Ruas,Paulo M.; Marin-Morales,Maria A.; Ruas,Claudete F.; Nakajima,Jimi N..
Karyotypes of six species of the genus Stevia from Southern Brazil were studied, utilizing root tip metaphases. All species were diploid with 2n = 22 chromosomes. It was possible to identify each species by chromosome morphology. The basic chromosome number for Brazilian species of Stevia is X = 11. This number is also found in almost all South American species. We suggest that in Stevia there is an evolutionary trend toward chromosomal rearrangement, caused mainly by pericentric inversions. It was found that, in addition to aneuploidy and polyploidy, chromosomal rearrangements are common in the tribe Eupatorieae.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome; Stevia. Cav.; Southern Brazil.
Ano: 1996 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400013
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Chromosome studies in the genus Mikania (Asteraceae) Genet. Mol. Biol.
Ruas,Paulo M.; Ruas,Claudete F.; Maffei,Eliane M.D.; Marin-Morales,Maria A.; Aguiar-Perecin,Margarida L.R..
Tipo: Info:eu-repo/semantics/article
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400042
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Genetic diversity among maize (Zea mays L.) landraces assessed by RAPD markers Genet. Mol. Biol.
Carvalho,Valdemar P.; Ruas,Claudete F.; Ferreira,Josué M.; Moreira,Rosângela M.P.; Ruas,Paulo M..
The genetic relationships among 81 maize accessions consisting 79 landraces and two improved varieties, maintained by farmers in southern Brazil were investigated using Random Amplified Polymorphic DNA (RAPD). Thirty-two highly informative primers amplified 255 markers of which 184 (72.2%) were polymorphics. Based on the RAPD markers, a dendrogram was constructed using the UPGMA method. The range of genetic similarity was from 0.78 to 0.91. The molecular data grouped the accessions into two main clusters, which were correlated according to kernel colors. Small clusters were seen associated to characteristics, such as kernel morphology. The analysis of the molecular data revealed that maize management adopted by small-scale farmers has contributed to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Corn landrace; Genetic variability; Molecular genetic markers; RAPD.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200017
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Genetic relationship in Coffea species and parentage determination of interspecific hybrids using ISSR (Inter- Simple Sequence Repeat) markers Genet. Mol. Biol.
Ruas,Paulo M.; Ruas,Claudete F.; Rampim,Leandro; Carvalho,Valdemar P.; Ruas,Eduardo A.; Sera,Tumoru.
Inter-simple sequence repeat (ISSR) markers were used to evaluate genetic divergence among eight Coffea species and to identify the parentage of six interspecific hybrids. A total of 14 primers which contained different simple sequence repeats (SSR) were used as single primers or combined in pairs and tested for PCR amplifications. Two hundred and thirty highly reproducible fragments were amplified, which were then used to estimate the genetic similarity and to cluster the Coffea species and hybrids. High levels of interspecific genetic variation were revealed. The dinucleotide motif (GA)9T combined with other di- tri- and tetra-nucleotides produced a greater number of DNA fragments, mostly polymorphics, suggesting a high frequency of the poly GA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea; Coffee breeding; DNA markers; ISSR.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300017
Registros recuperados: 5
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